Part:BBa_K5335008:Design

SpyCatcher-Linker-Cry6Aa2-Linker-CPPs+SpyTag-6*His

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1517
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 1517
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1622
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1517
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1517
  Illegal AgeI site found at 692
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Carefully consider the sequence of fusion proteins, with CPPs and Spycatcher placed on either the C or N terminal of Cry6Aa2

Source

The gene sequence was obtained from relevant papers, and the gene was synthesized by Genescript